| 质粒名称: | pBAD miniSOG |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 91774 |
| 存储实验室: | Erik Rodriguez, Roger Tsien |
|
载体骨架基本信息 |
|
| 载体名称: | pBAD |
| 载体出品公司: | Life Technologies |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4000 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | mini Singlet Oxygen Generator,miniSOG |
| 物种来源: | A. thaliana (mustard weed); Evolved from Arabidopsis thaliana phototropin 2 |
| 插入大小: | 321 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | araBAD |
| 标签/融合蛋白: | Hexa-Histidine tag（主干上的C末端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BamHI (未破坏) |
| 3' 克隆位点: | EcoRI (未破坏) |
| 5' 测序引物 | pBAD Forward |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pBAD miniSOG was a gift from Erik Rodriguez & Roger Tsien (Addgene plasmid # 91774 ; http://n2t.net/addgene:91774 ; RRID:Addgene_91774) |
| 参考文献部分: | A genetically encoded tag for correlated light and electron microscopy of intact cells, tissues, and organisms. Shu X, Lev-Ram V, Deerinck TJ, Qi Y, Ramko EB, Davidson MW, Jin Y, Ellisman MH, Tsien RY. PLoS Biol. 2011 Apr;9(4):e1001041. doi: 10.1371/journal.pbio.1001041. Epub 2011 Apr 5. 10.1371/journal.pbio.1001041 PubMed 21483721 |
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pBAD miniSOG载体 pBADminiSOG质粒基本信息
pBAD miniSOG质粒图谱 pBADminiSOG载体图谱和pBAD miniSOG载体序列 pBADminiSOG质粒序列多克隆位点信息
pBAD miniSOG质粒 pBADminiSOG载体简介
pBAD miniSOG质粒序列 pBADminiSOG载体序列
LOCUS pBAD miniSOG 4382 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Bacterial expression of a fluorescent protein, mini Singlet Oxygen
Generator (miniSOG).
ACCESSION .
VERSION .
KEYWORDS pBAD miniSOG
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4382)
AUTHORS Shu X, Lev-Ram V, Deerinck TJ, Qi Y, Ramko EB, Davidson MW, Jin Y,
Ellisman MH, Tsien RY
TITLE A genetically encoded tag for correlated light and electron
microscopy of intact cells, tissues, and organisms.
JOURNAL PLoS Biol. 2011 Apr;9(4):e1001041. doi:
10.1371/journal.pbio.1001041. Epub 2011 Apr 5.
PUBMED 21483721
REFERENCE 2 (bases 1 to 4382)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4382
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 16..300
/gene="araBAD"
/label=araBAD promoter
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
primer_bind 223..242
/label=pBAD Forward
/note="For vectors with E. coli araBAD promoter, forward
primer"
RBS 327..349
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 359..676
/codon_start=1
/product="mini singlet oxygen generator, a fluorescent
flavoprotein suitable for correlated light and electron
microscopy (Shu et al., 2011)"
/label=miniSOG
/translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
DG"
CDS 725..742
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(798..815)
/label=pBAD Reverse
/note="For vectors with E. coli araBAD promoter, reverse
primer"
terminator 968..1054
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 1146..1173
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 1192..1283
/gene="bla"
/label=AmpR promoter
CDS 1284..2144
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(1502..1521)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 2315..2903
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2804..2823
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 3057..3074
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 3089..3229
/label=bom
/note="basis of mobility region from pBR322"
primer_bind complement(3155..3174)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 3315..3337
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
CDS complement(3493..4371)
/codon_start=1
/gene="araC"
/product="L-arabinose regulatory protein"
/label=araC
/translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
EKVNDVAVKLS"
ORIGIN
1 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
61 tcttttactg gctgttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt
121 aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
181 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
241 ccataagatt agccgatcct acctgacgct ttttatcgca actctctact gtttctcgat
301 acccgttttt ttgggctaga aataattttg tttaacttta agaaggagat atggatccat
361 ggaaaaaagc ttcgtgatta ctgacccgcg cctgccggat aatccgatca tcttcgcctc
421 cgatggcttc ctggaactga ctgaatacag ccgtgaggaa attctgggtc gtaatggccg
481 tttcctgcag ggcccggaaa ccgatcaagc aactgttcag aaaattcgtg atgcaatccg
541 tgatcaacgt gaaatcaccg tgcagctgat taactatacc aaaagcggca agaaattctg
601 gaacctgttg cacctgcagc cgatgcgcga tcagaaaggt gagctgcagt acttcatcgg
661 ggttcagctg gatggtgaat tcatccctaa ccctctcctc ggtctcgatt ctacgcgtac
721 cggtcatcat caccatcacc attgagttta aacggtctcc agcttggctg ttttggcgga
781 tgagagaaga ttttcagcct gatacagatt aaatcagaac gcagaagcgg tctgataaaa
841 cagaatttgc ctggcggcag tagcgcggtg gtcccacctg accccatgcc gaactcagaa
901 gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc atgcgagagt agggaactgc
961 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
1021 tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ggagcggatt tgaacgttgc
1081 gaagcaacgg cccggagggt ggcgggcagg acgcccgcca taaactgcca ggcatcaaat
1141 taagcagaag gccatcctga cggatggcct ttttgcgttt ctacaaactc ttttgtttat
1201 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
1261 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
1321 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
1381 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
1441 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
1501 tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca
1561 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
1621 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
1681 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
1741 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
1801 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
1861 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
1921 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
1981 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
2041 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
2101 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
2161 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
2221 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
2281 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
2341 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
2401 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
2461 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
2521 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
2581 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
2641 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
2701 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
2761 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
2821 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
2881 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
2941 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
3001 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
3061 agtcagtgag cgaggaagcg gtagagcgcc tgatgcggta ttttctcctt acgcatctgt
3121 gcggtatttc acaccgtata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
3181 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc
3241 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
3301 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
3361 cgcgaggcag cagatcaatt cgcgcgcgaa ggcgaagcgg catgcataat gtgcctgtca
3421 aatggacgaa gcagggattc tgcaaaccct atgctactcc gtcaagccgt caattgtctg
3481 attcgttacc aattatgaca acttgacggc tacatcattc actttttctt cacaaccggc
3541 acggaactcg ctcgggctgg ccccggtgca ttttttaaat acccgcgaga aatagagttg
3601 atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc atccgggtgg tgctcaaaag
3661 cagcttcgcc tggctgatac gttggtcctc gcgccagctt aagacgctaa tccctaactg
3721 ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg cgacgctggc
3781 gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct cgcgtacccg
3841 attatccatc ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca gtaacaattg
3901 ctcaagcaga tttatcgcca gcagctccga atagcgccct tccccttgcc cggcgttaat
3961 gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc gaaagaaccc
4021 cgtattggca aatattgacg gccagttaag ccattcatgc cagtaggcgc gcggacgaaa
4081 gtaaacccac tggtgatacc attcgcgagc ctccggatga cgaccgtagt gatgaatctc
4141 tcctggcggg aacagcaaaa tatcacccgg tcggcaaaca aattctcgtc cctgattttt
4201 caccaccccc tgaccgcgaa tggtgagatt gagaatataa cctttcattc ccagcggtcg
4261 gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc gttaaacccg ccaccagatg
4321 ggcattaaac gagtatcccg gcagcagggg atcattttgc gcttcagcca tacttttcat
4381 ac
//
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